Linear Plasmid DNA

Linearized plasmid DNA (pDNA) serves as a crucial starting material for mRNA therapeutic developers. RNA is synthesized from DNA templates through the process of in vitro transcription (IVT). The design of these templates is vital, as it significantly influences the efficiency of transcription, as well as the quality and yield of the resulting mRNA. VGXI’s Linear pDNA service ensures optimal template design to maximize transcription efficiency and to produce high-quality linearized pDNA tailored to meet the demands of therapeutic research and production.

Benefits:

Robust Scale-Up Process

• • • VGXI excels at scaling up projects with a consistent manufacturing process to ensure seamless transitions from pre-clinical to bulk production.

Rigorous Quality Metrics

• • • The quality of the linear pDNA template impacts the quality of mRNA production. VGXI’s reliable QC panel meets the rigorous quality standards required for GMP production.

Client-Focused Expertise

• • • VGXI has over 20 years of experience communicating with clients to meet their specific pDNA manufacturing needs.

Linear Production Service Features

• • • • Up to 1500L fermentation scale
      • 1g/L plasmid DNA concentrations
      • Fast turnaround time & delivery
      • 100% of high-quality purified plasmid delivered
      • Optional: cell line, process development, and optimization services

Linear Plasmid QC Release Testing

• • • • Identity
      • Purity
      • Nucleic Acid Concentration
      • Structural Integrity (% supercoiled DNA)
      • Endotoxin
      • % of residual host components (genomic DNA, RNA, and protein)
      • pH
      • Poly(dA:dT) Tract Testing

All final material is delivered with a Certificate of Analysis and is ready for use in your application.